XPRESS
XPEDITE
XPERT
- Over 150 off-the-shelf targets from a wide variety of classes
- 2 months turn-around time
- Reproduction of previously published structures
- 2-4 months turn-around time
- Challenging projects, de novo structure determination, protein protein and protein-nucleic acid complexes, membrane proteins
- Average 4 months turn-around time
XPRESS
Targets
Examples
Kinases
AMP kinase, CDC7-DBF4, CDK1-CKS1, CDK1-CKS2-CCNE2, CDK2, CDK2-CCNA2, CDK2-CCNE2, CDK9-CCNT1,
CDK12-CCNK2, CK1a, CK1d, DYRK1A, EGFR, FGFR3, FGFR4, FLT3, FMS1, IRAK4, JAK1, JAK2, JNK1, JNK2,
JNK3, LCK, MAP4K1, MKNK2, PIK3CA-PIK3R1, PIP4K2A, PIP4K2B, PIP4K2C, PKA, PKR, RET, SIK3, SRC,
TGFBR1, TGFBR2, Wee1 and more
Proteases
3C-like protease (SARS-CoV-2 and Norovirus), Cathepsin K, Cathepsin S, MALT1, USP7, USP21 and more
Targeted Protein
Degradation
Degradation
SKP1-SKP2-CKS1, VHL-Elongin B-Elongin C and more
Epigenetic Targets
SBRD4, BRD9, CREBBP, HDAC2, HDAC7, HDAC8, JMJD2C, JMJD2D, MLLT1, NSD1, SIRT2 and more
Innate Immunity
cGAS, STING and more
Nuclear Receptors
ERa, GR, RORg and more
Nucleic acid binding
proteins
proteins
APOBEC3B, cGAS, PARP1, SMARCA2, SND1, STAT6 and more
XPEDITE
XPEDITE portfolio
Is your target protein of interest not present in our XPRESS list even though there are several
published structures available? Our XPEDITE service aims to provide you with rapid access to crystal
structures of previously crystallized constructs with your ligands of interest. As part of this
service, we can also produce alternative constructs containing disease-related mutations or further
optimize established crystal systems in order to determine structures at higher resolution.
- Established and/or published conditions
- 2-4 months turn-around
XPERT
XPERT service
Customized Structural Biology Solutions
Under XPERT services we offer customized structural biology solutions for your challenging targets, using either crystallography, Cryo-EM or NMR.
Our team of experts will work closely with you to prepare a sound scientific plan with clear milestones to address your needs and lead you to successful de novo structure determination. This blueprint is then translated into a flexible and success-based payment plan, which is accompanied by personalized project management.
- De novo structure determination
- Challenging targets
- Average 4 months turn-around
Facilities
Sample Characterization
Standard protein QC
- SDS-PAGE
- HPLC
- MS (intact and fingerprinting)
- DLS
- nanoDSF
- MST
- SPR
- SEC-RALS
Liquid Handling
STP Labtech Mosquito LCP crystallization robots
- 48 and 96 well trays
- Sitting-drop, hanging-drop & microbatch formats
- Setup for LCP membrane protein crystallization
Apricot S2 Pipettor
ARI Scorpion
- High-throughput creation of custom screens
Incubation & Observation
Formulatrix ROCK IMAGER plate hotels with UV imaging
- 4, 12 and 20°C
- 1000 plates per temperature
Zeiss V20 microscopes
Crystal Optimization
Humidity Controller
Crystal Testing
Bruker MetalJet
- World’s most intense in-house X-ray source
- Photon III detector
- Scout automatic sample changer
- ISX stage for in situ diffraction
- High throughput testing of crystals
- Instant in-house data collection at resolutions that
match synchrotron data in many cases