High accuracy
High sensitivity
Hign recovery
CHO Host Cell Protein ELISA Kit
This kit is used for determining the presence of Chinese Hamster Ovary (CHO) protein impurities in products manufactured by recombinant expression in CHO host cells. The kit is for Research and Manufacturing Use Only.
How does it work ?
The CHO assay is a double antibody sandwich ELISA assay. Samples containing CHO HCPs are reacted simultaneously with an affinity purified capture anti-CHO antibody (goat polyclonal) and a horseradish peroxidase (HRP) enzyme labeled anti-CHO antibody. The immunological reactions result in the formation of a sandwich complex of solid phase antibody-HCP-enzyme labeled antibody. The plate are washed to remove any unbound reactants. The substrate TMB is then reacted. The amount of hydrolyzed substrate is read on a microplate reader and is directly proportional to the concentration of CHO Host Cell Proteins present.
What's inside
Component Specification Stored
Anti-CHO antibody coated plate 12x8 well 2~8°C
CHO HCPs Standards
(0, 1, 3, 10, 40 and 100ng/mL)
0.5mL/vial 2~8°C
Anti-CHO antibody conjugated HRP 10 L/vial 2~8°C
10 × Wash solution 1x60mL 2~8°C
Substrate A 1x10mL 2~8°C
Substrate B 1x10mL 2~8°C
Stop Solution 1x10mL 2~8°C
Handbook 1 sheet
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