OVERVIEW
All lanes : Anti-LAPTM4B-4 Antibody at 1:250 dilution Lane 1: Hela whole cell lysates Lane 2: MCF-7 whole cell lysates Lane 3: NCI-H460 whole cell lysates Lane 4: MDA-MB453 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 41 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
OVERVIEW
Western blot analysis of lysates from Hela, MCF-7 cell line, rat cerebellum tissue lysate(from left to right), using LAPTM4B Antibody (4)(Cat. #AP20871a). AP20871a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.