TBB5 Antibody
Mouse Monoclonal Antibody (Mab)
Western blot analysis of lysates from 293, CEM, Hela, Y79, rat PC-12 cell line, mouse brain tissue lysate(from left to right), using TBB5 Antibody(Cat. #AM1031a). AM1031a was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20μg per lane.
Immunohistochemical analysis of paraffin-embedded H.kidney section using TBB5 Antibody(Cat#AM1031a). AM1031a was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Immunofluorescent analysis of A549 cells, using TBB5 Antibody (Cat. #AM1031a). AM1031a was diluted at 1:25 dilution. Dylight Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor® 554 (red) conjugated Phalloidin (red).
Formalin-fixed and paraffin-embedded human testis reacted with TBB5 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Product info
Application IHC-P,WB,IF,E
Primary Accession P07437
Reactivity Human, Mouse, Rat
Predlicted Bovine, Chicken, Hamster, Pig, Rat, Xenopus
Host Mouse
Clonality Monoclonal
Isotype IgG2b,Igκ
Clone Names 87CT59.3.7
Calculated MW 49671 Da
Additional info
Gene ID 203068
Other Names Tubulin beta chain, Tubulin beta-5 chain, TUBB, TUBB5
Target/Specificity TBB5 recombinant protein is used to produce this monoclonal antibody.
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