WuXi AppTec | Discovery Biology

Established Assays

Optimized assay conditions

Flexible

Single/multiple assay in parallel

Cost-effective

Low budget, high quality data

Fast Turnaround Time

Up to 2 weeks

Procedures

Protein Supply

Established protein construct in house
Tailored high quality assay grade proteins
Protein QC: SDS-PAGE, aSEC, MS analysis included

Assay Initiation & Measurements

Established assay conditions
Established labeling/coupling method
Published tool compounds Up to 3 Tms / Kds / IC50s included

Results & Reports

A tabulated report included
Extended report available including measured curves, assay conditions, analysis and scientific summary in pdf-format

Assay List

Target	PP	MST	nanoDSF	SPR	Biochemical Assay	Xtal
ACSS2	✔	✔	✔	✔		✔
APOBEC3B	✔				✔	✔
ASGR1	✔				✔	✔
AURKA	✔	✔	✔		✔	✔
BDR4	✔				✔	✔
b-Raf	✔				✔	✔
BTK	✔		✔	✔		✔
BTK	✔		✔			✔
CDK2	✔	✔		✔	✔	✔
CDK2/CCNA2	✔	✔	✔	✔	✔	✔
CDK2/CCNE1	✔	✔	✔	✔	✔	✔
CDK7	✔	✔	✔
CK1d	✔				✔	✔
CLK1	✔				✔	✔
CRBN/DDB1	✔	✔	✔
cREL	✔	✔	✔
DHODH	✔				✔	✔
DOCK5	✔	✔	✔		✔
EGFR	✔	✔	✔		✔	✔
GLUT1	✔	✔	✔
GRB2	✔	✔	✔
Hsp90	✔				✔	✔
IRAK4	✔		✔			✔
ITCH	✔		✔	✔
KARI	✔	✔	✔
KIT	✔	✔	✔
KLHDC2	✔	✔	✔		✔
KRAS	✔	✔	✔	✔	✔	✔
MALT1	✔	✔	✔	✔		✔
MAP4K1	✔	✔	✔		✔	✔
MCL1	✔				✔	✔
MEK-1	✔				✔	✔
Mpro	✔				✔	✔
NEDD4	✔		✔		✔
NLRP3	✔	✔	✔	✔	✔
NRAS	✔	✔	✔	✔	✔
P2RX3	✔	✔	✔
p38	✔	✔	✔
p38a	✔	✔	✔	✔	✔	✔
PAH	✔				✔	✔
PD-L1	✔			✔		✔
PIK3CA	✔	✔	✔	✔	✔	✔
PIK3CA/PIK3R1	✔	✔	✔	✔	✔	✔
PIM2	✔				✔	✔
PIP4K2B	✔				✔	✔
PIP4K2C	✔				✔	✔
PLK1	✔				✔	✔
POLQ	✔	✔	✔	✔
PRMT5/WDR77	✔	✔	✔		✔
PTPN11	✔	✔	✔		✔	✔
Rac1	✔	✔	✔
SHP2	✔	✔	✔			✔
SIK3	✔	✔	✔		✔
SMARCA2	✔	✔	✔		✔	✔
SOS1	✔	✔	✔		✔	✔
SPSB2	✔	✔	✔
Src	✔				✔	✔
STAT3	✔			✔
STAT4	✔			✔
STAT6	✔	✔	✔	✔	✔	✔
STING	✔	✔	✔	✔	✔	✔
TNKS1	✔				✔	✔
USP5	✔	✔	✔	✔
USP7	✔	✔	✔	✔	✔	✔
VHL/TCEB1/TCEB2	✔	✔	✔	✔	✔	✔
Wee1	✔	✔	✔
WRN	✔	✔	✔	✔	✔

Protein Show Cases

WT SMARCA2 vs. BRM/BRG1 ATP Inhibitor-1

Prime Protein
Production

Biophysical Assay

Biochemical Assay

PolQ – Polymerase domain vs. 5‘ Overhang DNA

Prime Protein
Production

Biophysical Assay

NLRP3 PYD vs. MCC-950

Prime Protein
Production

Biophysical Assay

Biochemical Assay

Protein List

Target Name	Uniprot ID	Tag	Boundary	Molecular Weight (kDa)
ACSS2	Q9NR19	NA	Individual Domains	73.5
ACSS2	Q9NR19	Avi, His	Individual Domains	77.0
AKT1	P31749	His	Individual Domains	39.5
AKT1	P31749	NA	Individual Domains	51.7
ARHGEF11	O15085	Sumo, His	Individual Domains	67.5
ARHGEF5	Q12774	Sumo, His	Individual Domains	65.9
ASB9	Q96DX5	His	Individual Domains	29.4
ATP13A2	Q9NQ11	NA	Individual Domains	129
AURKA	O14965	His	Full Length	46.7
AURKA	O14965	His	Individual Domains	34.6
BIRA	P06709	His	Full Length	37.8
BIRC2	Q13490	His	Individual Domains	13.1
BIRC3	Q13489	His	Individual Domains	13.1
BLM	P54132	Avi, His, Flag	Individual Domains	80.6
BRD2	P25440	NA	Individual Domains	14.5
BRD4	O60885	Avi, His	Individual Domains	19.6
BRD4	O60885	mSA, His	Individual Domains	29.6
BRD4	O60885	His	Individual Domains	47.4/17.5
BRD4	O60885	Halo, His	Individual Domains	51.9
BRD4	O60885	FcSA	Individual Domains	54.8
BRD4	O60885	NA	Individual Domains	15.1
BTK	Q06187	NA	Full Length	76.3/76.5
BTK	Q06187	His	Individual Domains	34.0
BTK	Q06187	NA	Individual Domains	31.8
BTK	Q06187	mSA2, His	Full Length	92.3
BTK	Q06187	His	Full Length	31.8
CAMK4	Q16566	Sumo, His	Individual Domains	50.1
CAMKK2	Q96RR4	His	Individual Domains	34.5
CBL	P22681	His	Individual Domains	46.3
CBLB	Q13191	His	Individual Domains	47.1
CCL2	P13500	Sumo, His	Individual Domains	22.3
CCNA2	P20248	NA	Individual Domains	29.9
CCNA2	P20248	Avi, His	Full Length	51.6
CCNA2/CDK2 Complex	P24941, P20248	Avi, His	Full Length	85.8
CCNB1	P14635	NA	Individual Domains	31.1
CCNE1	P24864	NA	Individual Domains	33.0
CCNE1	P24864	Avi, His	Full Length	51.6
CD19	P15391	His	Individual Domains	29.68
CD47	Q08722	His	Individual Domains	15.3
CDC20	Q12834	NA	Individual Domains	34.9
CDK1	P06493	Avi	Full Length	36.1
CDK1	P06493	NA	Full Length	35.2
CDK12, CyclinK	Q9NYV4, O75909	His	Individual Domains	74.7
CDK13, CyclinK	Q14004, O75909	His, Gst	Individual Domains	104.1
CDK2	P24941	His, Mbp	Full Length	78.2
CDK2	P24941	Avi	Full Length	36.1
CDK2	P24941	NA	Full Length	34.0
CDK2, CCNA2	P24941, P20248	NA	Individual Domains	63.9
CDK2, CCNE1	P24941, P24864	NA	Individual Domains	67.0
CDK2, CCNE1	P24941, P24864	NA	Individual Domains	77.0
CDK2, CCNE1	P24941, P24864	Avi, His	Full Length	84.3
CDK4, CyclinD3	P11802, P30281	His, Gst	Full Length	95.1
CDK7	P50613	His	Individual Domains	35.2
CDK7, cyclin H1, MAT(FL)	P50613, P51946, P51948	His, Gst	Individual Domains	141.5
CFB	P00751	NA	Individual Domains	33.0
CRBN, DDB1	Q96SW2, Q16531	Avi, His	Individual Domains	177.4
CRBN, DDB1	Q96SW2, Q16531	Avi	Individual Domains	175.2
CRBN, DDB1	Q96SW2, Q16531	NA	Individual Domains	173.5
CRBN, DDB1	Q96SW2, Q16531	His	Individual Domains	175.8
CRBN/DDB1 complex	Q96SW2, Q16531	Avi, His	Full Length	180.7
CRBN/DDB1 complex	Q96SW2, Q16531	NA	Individual Domains	140.1
CREBBP	Q92793	NA	Individual Domains	14.2
cREL	Q04864	His	Individual Domains	37.0
CSNK1G2	P78368	Sumo, His	Individual Domains	49.8
DCAF15, DDA1, DDB1	Q66K64, Q9BW61, Q16531	His	Individual Domains	206.3
DDB1	Q16531	His	Full Length	127.9
DDR1	Q08345	NA	Individual Domains	35.5
DHFR	P00374	His	Full Length	23.6
DTYMK	P23919	Sumo, His	Full Length	37.4
EGFR	P00533	Sumo, His, Avi	Individual Domains	53.3
EGFR	P00533	NA	Individual Domains	37.5
EGFR	P00533	Halo, His	Individual Domains	75.2
EGFR	P00533	His, mSA2	Individual Domains	53.6
EGFR	P00533	His	Individual Domains	39.5
ESR1	P03372	NA	Individual Domains	29.6
EXOSC10	Q01780	Sumo, His	Individual Domains	63.7
FBXO44	Q9H4M3	His	Full Length	30.8
FBXW7	Q969H0	His	Individual Domains	68.0
FGFR2	P21802	His	Individual Domains	38.2
FGFR3	P22607	His	Individual Domains	36.6
FGFR3	P22607	His	Individual Domains	33.7
FGFR4	P22455	His	Individual Domains	34.8
FGFR4	P22455	His	Individual Domains	33.8
folA	P0A547	NA	Full Length	18.2
GFP	P42212	His	Full Length	29.0
GLUT1	P11166	His	Full Length	55.7
GRB2	P62993	NA	Full Length	25.4
GRB2	P62993	His	Full Length	27.6
GSK3	P49841	NA	Individual Domains	43.3
HDAC8	Q9BY41	His	Full Length	43.1
HECTD3	Q5T447	His	Individual Domains	98.1
HER2	P04626	His	Individual Domains	38
HK2	P52789	Sumo, His	Individual Domains	113.96
HMOX1	P09601	Avi	Full Length	34.8/34.83
HMOX2	P30519	Avi, His	Individual Domains	28.1
HSD17B13	Q7Z5P4	His	Individual Domains	28.4
IRAK3	Q9Y616	His	Individual Domains	35.6
IRAK4	Q9NWZ3	Avi	Individual Domains	36.2
IRAK4	Q9NWZ3	His	Individual Domains	36.9
IRAK4	Q9NWZ3	NA	Individual Domains	33.9
IRF4	Q15306	Flag	Full Length	52.7
IRF4	Q15306	Flag	Individual Domains	27.8
KCTD5	Q9NXV2	His	Full Length	27.1
KCTD8	Q6ZWB6	Sumo, His	Individual Domains	28.3
KEAP1	Q14145	His	Full Length	70.7
KEAP1	Q14145	Mbp, Sumo, Flag, His	Full Length	127.7
KHK	P50053	Sumo, His	Individual Domains	45.6
KIT	P10721	NA	Individual Domains	38.2
KLHDC2	Q9Y2U9	Avi, His	Individual Domains	45.5
KLHDC2	Q9Y2U9	His	Individual Domains	43.5
KLHL40	Q2TBA0	His	Full Length	70.3
KLHL40	Q2TBA0	Mbp, Sumo, Flag, His	Full Length	127.3
KRAS	P01116	His	Individual Domains	21.5
KRAS	P01116	His	Individual Domains	22.2
KRAS	P01116	NA	Individual Domains	19.3
KRAS	P01116	His	Individual Domains	21.8
KRAS	P01116	Avi, His	Individual Domains	23.2
KRAS	P01116	Avi	Individual Domains	21.1
LacZ	P00722	His	Individual Domains	118.3
LYK5	Q7RTN6	Sumo, His	Individual Domains	55.2
LYN	P07948	His, Gst	Full Length	87.2
MALT1	Q9UDY8	NA	Individual Domains	43.9
MAP2K6	P52564	Sumo, His	Individual Domains	45.9
MAPK10	P53779	Sumo, His	Individual Domains	55.3
MAPK12	P53778	Sumo, His	Individual Domains	54.8
MAPK13	O15264	His	Individual Domains	42.8
MAPK14	Q16539	Avi, His	Full Length	44.6
MAPK14	Q16539	NA	Full Length	41.7
MAPK14	Q16539	His	Full Length	44.6
MAPK14	Q92918	NA	Individual Domains	34.4
MAPK3	P27361	Sumo, His	Full Length	56.7
MAPK6	Q16659	His	Individual Domains	38.5
MAPK6	Q16659	His	Individual Domains	38.4
MAPK8	P45983	Sumo, His	Individual Domains	55.6
MAPK9	P45984	Sumo, His	Individual Domains	56.8
MAPKAPK3	Q16644	Sumo, His	Individual Domains	49.8
MARK3	P27448	Sumo, His	Individual Domains	50.7
Mb21d1	Q8C6L5	NA	Individual Domains	42.5
MDM2	Q00987	His	Individual Domains	13.4
MEK1	Q02750	His	Individual Domains	37.9
MEK1	Q02750	His	Individual Domains	41.1
MLLT1	Q03111	His	Individual Domains	18.3
MLLT1	Q03111	Avi, His	Individual Domains	20.2
Mpro	P0DTD1	NA	Individual Domains	33.9
Mpro	P0DTD1	His	Individual Domains	34.9
NEDD4	P46934	His, Gst	Individual Domains	87.9
NEDD4	P46934	His	Individual Domains	46.9
NEDD4L	Q96PU5	His	Individual Domains	112.9
NFKB1	P19838	His	Individual Domains	43.4
NLRP1	Q9C000	NA	Individual Domains	22.8
NLRP3	Q96P20	Mbp, Snap	Individual Domains	164.1
NLRP3	Q96P20	Mbp, His	Individual Domains	145.3
NLRP3	Q96P20	Mbp, Avi	Individual Domains	146.5
NME1	P15531	Sumo, His	Individual Domains	30.7
NME4	O00746	Sumo, His	Individual Domains	29.8
NRAS	P01111	Avi, His	Individual Domains	21.8
NRAS	P01111	NA	Individual Domains	18.8
NRAS	P01111	Avi, His	Individual Domains	22.7
p38 alpha	Q16539	Sumo, His	Full Length	54.8
p53	P04637	Avi, His	Individual Domains	28.1
PAK4	O96013	NA	Individual Domains	33.1
PAPSS2	O95340	Sumo, His	Individual Domains	35.5
PARP1	P09874	Avi, His	Individual Domains	45.1
PARP2	Q9UGN5	Avi, His	Full Length	69.9
PARP2	Q9UGN5	Avi, His	Individual Domains	45.1
PD1L1	Q9NZQ7	His	Individual Domains	14.8
PFKFB3	Q16875	Sumo, His	Individual Domains	73.1
PFKFB3	Q16875	Sumo, His	Individual Domains	64.9
PHF2	O75151	Sumo, His	Individual Domains	58.8
PIK3C3	Q8NEB9	Sumo, His	Individual Domains	81.9
PIK3CA	P42336	His	Individual Domains	112.1
PIK3Ca/PIK3R1	P42336, P27986	His	Full Length	210.0
PIK3Ca/PIK3R1 complex	P42336, P27986	His	Individual Domains	159.4
PIK3Ca/PIK3R1 complex	P42336, P27986	Avi	Individual Domains	160.3
PIM1	P11309	Gst	Full Length	62.0
PIM2	Q9P1W9	Gst	Full Length	60.5
PIM3	Q86V86	Gst	Full Length	62.2
PIM3	Q86V86	NA	Full Length	35.9
PIP4K2B	P78356	NA	Individual Domains	44.3
PKM2	P14618	His	Individual Domains	59.7
PLK1	P53350	Sumo, His	Individual Domains	40.3
PLK1	P53350	NA	Individual Domains	37.5
PLK1	P53350	His	Individual Domains	37.7
PLK1	P53350	Gst, His, Avi	Full Length	98.5
PLK2	Q9NYY3	His	Individual Domains	35.3
PLK3	Q9H4B4	Gst	Individual Domains	58.8
POLQ	O75417	His	Individual Domains	95.3
PRMT2	P55345	NA	Individual Domains	46.1
PRMT5, MEP50	O14744, Q9BQA1	Flag/His	Individual Domains	111.4
PRMT5/MEP50 complex	O14744, Q9BQA1	Avi, His, Flag	Full Length	453.2
PRMT6	Q96LA8	NA	Full Length	42
PRMT7	Q9NVM4	NA	Full Length	78.5
PTGS2	P35354	His, Flag	Full Length	70.8
PTPN11	Q06124	His	Individual Domains	64.5
PTPN11	Q06124	His	Individual Domains	61.7
RAF1	P04049	Gst	Individual Domains	35.7
RET	P07949	NA	Individual Domains	35.7
RIOK2	Q9BVS4	Sumo, His	Individual Domains	50.6
RIPK1	Q13546	NA	Individual Domains	49.7/37.4
RIPK2	O43353	NA	Individual Domains	34.4
RN114	Q9Y508	His	Full Length	27.3
ROR1	Q01973	Avi	Individual Domains	36.8
RPS6KA5	O75582	Sumo, His	Individual Domains	52.3
RPS6KA6	Q9UK32	Sumo, His	Individual Domains	48.4
RSK1	Q15418	NA	Full Length	82.8
RSK2	P51812	NA	Full Length	83.8
RSK3	Q15349	NA	Full Length	83.3
RSK4	Q9UK32	NA	Full Length	83.9
SIAH1	Q8IUQ4	His	Individual Domains	23.2
SIAH2	O43255	His	Individual Domains	22.9
SIAH2	O43255	His	Full Length	35.7
SIK3	Q9Y2K2	His	Individual Domains	38.5
SKP1	P63208	His	Full Length	19.1
SMARCA2	P51531	Avi, His	Individual Domains	73.1
SMARCA2	P51531	His	Individual Domains	93.7
SMARCA4	P51532	His	Individual Domains	96.6/99.9
SORT1	Q99523	Avi, His	Individual Domains	80.4
SOS1	Q07889	His	Individual Domains	59.4
SOS1	Q07889	NA	Individual Domains	57.2
SOS1	Q07889	Flag	Individual Domains	58.5
SPOP	O43791	His	Full Length	43.8
SPOP	O43791	His	Individual Domains	37.3
SPSB2	Q99619	His	Individual Domains	23.8
STAT3	P40763	Avi	Individual Domains	70.1
STAT3	P40763	Avi, His	Full Length	92.1
STAT3	P40763	His	Individual Domains	70.7
STAT6	P42226	Avi	Individual Domains	73.43
STAT6	P42226	NA	Individual Domains	60.1
STING	Q86WV6	NA	Individual Domains	22.4
STING	Q86WV6	Avi, Flag, His	Individual Domains	32.3
STK38	Q15208	Sumo, His	Individual Domains	52.2
T4 DNA ligase	P00970	NA	Full Length	55.3
TAF1	P21675	His	Individual Domains	33.4
TCEB1(ELOC), TCEB2(ELOB)	Q15370, Q15369-2	His	Individual Domains	25.0
TCEB1(ELOC), TCEB2(ELOB), VHL	P40337, Q15370, Q15369-2	NA	Individual Domains	18.7/10.8/11.7
TCEB1(ELOC), TCEB2(ELOB), VHL	P40337, Q15370, Q15369-2	His/Avi/NA	Individual Domains	20.9/13.7/11
TDO2	P48775	NA	Individual Domains	44.8
TDO2	P48775	His	Individual Domains	45.2
TEAD2	Q15562	His	Individual Domains	27.0
TEV	P04517	Gst	Individual Domains	53.5
TEV	P04517	His	Individual Domains	28.6
TFR1	P02786	Avi	Individual Domains	77.8
TFR1	P02786	Avi	Individual Domains	77
TFR1	P02786	His	Individual Domains	75.99
TGFBR1	P36897	Gst	Individual Domains	61.1
TNKS1	O95271	NA	Individual Domains	26.8
TNKS2	Q9H2K2	NA	Individual Domains	21.8
TYK2	P29597	Avi	Individual Domains	37.0
TYK2	P29597	His	Individual Domains	35.9
TYK2	P29597	Avi, His	Individual Domains	36.5
UBE2D2	P62837	His	Full Length	17.7
UBE3A	Q05086	His	Full Length	101.7
UCK2	Q9BZX2	Sumo, His	Individual Domains	42.8
ULP1	Q02724	His	Individual Domains	26.3
ULP1	Q02724	His	Individual Domains	27.3
USP7	Q93009	NA	Full Length	128.7
USP7	Q93009	His, Gst	Individual Domains	69.5
USP7	Q93009	NA	Individual Domains	43.4
VHL	P40337	His	Full Length	25.2
WEE1	P30291	His	Individual Domains	32.4
WNK1	Q9H4A3	Sumo, His	Individual Domains	45.4
WRN	Q14191	Flag, His	Individual Domains	84.7
WRN	Q14191	His	Individual Domains	48.9
WRN	Q14191	Avi, His	Individual Domains	69.7
WRN	Q14191	Avi, His	Individual Domains	85.6
WWP1	Q9H0M0	His	Full Length	106.3
XIAP	P98170	His	Individual Domains	15.3
ZBTB7A	O95365	Avi	Individual Domains	16.0

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